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Asthmatic HBECs exhibit significantly larger KCa3.1 currents than healthy HBECs.

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posted on 2015-12-23, 10:15 authored by Greer K. Arthur, S. Mark Duffy, Katy M. Roach, Rob A. Hirst, Aarti Shikotra, Erol A. Gaillard, Peter Bradding

(A) Current-voltage curves demonstrating that baseline whole cell membrane currents recorded from asthmatic and healthy primary HBECs were similar. (B) The 1-EBIO-dependent (1-EBIO minus baseline) currents recorded from asthmatic HBECs (n = 29 cells from 8 donors) were significantly larger than those from healthy controls (n = 16 cells from 5 donors); *P = 0.006 at +40 mV. (C) Raw 1-EBIO-dependent current trace demonstrating characteristic features of KCa3.1. The voltage protocol is shown inset. The KCa3.1 channel blocker TRAM-34 (200 nM) inhibited currents induced by 1-EBIO in both (D) asthmatic HBECs (n = 19 cells from 8 donors) and (F) healthy HBECs (n = 14 cells from 5 donors). Data are presented as mean ± SEM.

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    KCa 3.1 blockadeKCa 3.1 ion currentsKCa 3.1 blockersPrimary HBECsepithelial cell proliferationtgfepithelial fluid secretionKCa 3.1 mRNAHBEC functionKCa 3.1 currentsemtH 292 cellsairway epithelial biologySelective KCa 3.1 blockerstramHuman Bronchial Epithelial Cellsneoplastic H 292 epithelial cell lineKCa 3.1 protein expressionicaKCa 3.1 channelbeasKCa 3.1 K

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