Assessment of astrocyte viability in response to 12 hours of oxygen- glucose deprivation.

<p>(<b>1A</b>) Astrocyte viability was assessed using an MTT assay and did not show a difference in absorbance between the astrocytes exposed to OGD-alone compared to the astrocytes that were exposed to OGD with relaxin-2 (10, 50 ng/mL), relaxin-3 (10, 50 ng/mL) or R3/I5 (10, 50 ng/mL). (<b>1B</b>) Astrocytes that were exposed to relaxin-2, relaxin-3, and R3/I5 during the 24 hour OGD protocol all demonstrated an increased viability when compared to untreated astrocytes that were exposed to OGD for 24 hours. (<b>1C</b>) Finally, astrocytes that were exposed to relaxin-2, relaxin-3, and R3/I5 during the 48 hour OGD protocol did not exhibit different viability or mitochondrial uptake of MTT compared to untreated astrocytes that were exposed to OGD. The * indicates significantly different from OGD astrocytes.</p>