Apical CFTR expression in non-CF cells compared to CF cells.

Ezrin and E-cadherin positive cells were analyzed for apical CFTR expression per individual by all observers and the mean percentage of cells displaying apical CFTR was plotted. A. Differences in distribution for the proportion of columnar epithelial cells expressing apical CFTR in non-CF individuals (n = 17) compared to individuals with CF homozygous for F508del-CFTR using Student’s t-test. Apical CFTR expression levels for CF patients with other mutations are displayed at the right scatter plot. A subject that expresses a CFTR allele associated with reduced gating is indicated in green (F508del/S1251N). Repetitive measurements of a single subject are indicated in red.