Antibodies against HLA/MICA antigens in pre- and post-transplant serum samples.

IgG antibodies against HLA class I and II antigens and MICA antigens were measured by single antigen Luminex beads arrays. MFI values represent the quantitation of alloantibodies detected. (A) The donor’s and recipient’s HLA antigen types and the positive reacted HLA antigens (others) with test serum samples are labeled as grouped.The MFI values of antibodies detected from patient serum before (gray bars) and after the first transplant (solid bar) are given. (B) Anti-MICA antibodies were detected using 11 common MICA antigens. MICA001, MICA002, MICA007, MICA012, MICA017, and MICA018, all from the MICA-G1 antigen group were present in pre- and post-first transplant patient sera; other antigens tested were negative. DSA to the antigen produced by MICA*018 allele is indicated with an arrow. C1q-PE, rather than goat anti human IgG conjugated with PE, was used to demonstrate the complement fix (C1q, white bars).