Antibiotic resistance, phage susceptibility and geographic/ecological metadata of Achromobacter strains.

(A) Quantitative display of susceptibility of 62 strains (rows on the y-axis) to 35 antibiotics (columns on the x-axis) shown as heatmap of antibiotic susceptibility values using Euclidian distance and Ward clustering for both rows and columns as implemented in the heatmap.2() function of the R gplots package [77]. The colour code of the heatmap is given in the upper left corner of the heatmap. The more red the colour, the more resistant is the strain to the antibiotic (for fields covered by the legend, all strains are fully resistant to the respective antibiotic). Only the row dendrogram is shown, which depicts clusters of strains with similar antibiotic resistance patterns (see main text). Antimicrobial agents are grouped according to their clusters of susceptibility patterns of the strains (column dendrogram is not shown). The names of the antibiotics are colored according to their chemical classification. The respective color legend is shown at the bottom of A. (B) Horizontal bar plot which depicts the sum of phages to which a given strain is susceptible (for details see section C). (C) Host range analysis of isolated phages (columns in C). The detailed results of susceptibility of strains per phage are shown as level plot with rows being ordered according to Fig. 2A. A grey color indicates resistance or immunity, blue color indicates plaque formation of a given phage (see the labeling of the x-axis of C) on a given strain (y-axis). The respective legend is shown in the upper right corner of C (for fields covered by the legend, phages do not propagate on the indicated strains). The order of phages (columns of levelplot in C) is based on the number of strains on which a given phage is able to propagate (indicative for the width of the host range). This number of strains is given in the vertical bar plot below the level plot. (D) Names of the Achromobacter strains and respective geographic and ecological metadata. Strain names were marked in colors according to clusters from MLSA (see Figure 1), metadata values are differently colored in order to facilitate readability (a colour legend is not separately given).