Anti-acetyl lysine and anti-succinyl lysine western blot analyses.

168 (WT), SS110 (ΔacuA), SS38 (ΔacuC ΔsrtN), SS51 (ΔackA), SS52 (Δpta), SS53 (ΔackA Δpta), and SS111 (ΔackA Δpta ΔacuA) strains were grown in modified Spizizen’s minimal medium supplemented 30 mM glucose or citrate. Cells were harvested when the OD₆₆₀ reached 0.5. Total lysates containing 20 μg of protein were separated by 10% SDS-PAGE. The signal intensity in each lane was quantified and was expressed relative to the value for the wild-type strain after subtracting the background. Upper panel: western blot with anti-acetyl lysine (A) and anti-succinyl lysine (B) antibodies; Lower panel: Ponceau staining.