Antagomir efficiency varies across immune cell populations.

Isolated lung cells were incubated with fluorescently (DY547)-labeled Ant at indicated concentrations for 1 hour. Mean fluorescent intensity and percentage of DY547⁺ cells for granulocytes (SSC^hi), B220⁺ and CD4⁺ cells (A). CD4⁺ T cells were CFSE-labeled and cultured with 5 μM Ant for 3 days. Representative histogram plots and proliferation analysis for unlabeled and unstimulated, CFSE-labeled controls (Controls), PBS-treated (PBS) and Ant-treated (either Scramble or Ant-155) CD4⁺ T cells (B). Representative graphs from 3 independent experiments. Error bars SEM (n = 3 replicates/group). Fluorescently-labeled Ant uptake by lung immune cells in vivo following intranasal Ant administration (C). Mean fluorescent intensity and percentage of DY547⁺ cells for CD11c⁺, F4/80⁺, Ly6G⁺, B220⁺ and CD4⁺ cell populations.