Ang II-mediated aortic superoxide and peroxynitrite generation in ACE2KO mice.

Representative dihydroethidium (A–D) and nitrotyrosine (E–H) fluorescence images, relative fluorescence values (I) and NADPH oxidase activity (J) exhibited aortic superoxide generation and nitrotyrosine formation and NADPH oxidase activities in WT and ACE2KO mice in response to Ang II. Representative Western blot analysis showed protein level of nitrotyrosine (K; 60 kD) in aortas of WT and ACE2KO mice infused with Ang II. R.E. = relative expression; KO, knockout; A.U., arbitrary units; NADPH, nicotinamide adenine dinucleotide phosphate; α-tubulin (55 Kd) was used as an endogenous control. n = 5−6. ^**P<0.01 compared with corresponding vehicle-treated group; #, P<0.05 compared with WT+Ang II group; φ, P<0.05 compared with WT+Vehicle group.