Anatabine reduces Bace1 mRNA expression in brain of Tg PS1/APPswe mice.

The histogram represents quantitative results from RT-qPCR analysis calculated using the Delta-Delta Ct Method. The means ± SEM Bace1 mRNA levels are expressed as a relative fold change compared to control (wild-type placebo). ANOVA shows a significant main effect of the genotype and treatments (P<0.009 and P<0.001) on Bace1 transcription. Post-hoc analyses reveal a significant difference in Bace1 mRNA levels between wild-type and Tg PS1/APPswe placebo mice (P<0.009) showing an upregulation of Bace1 mRNA in Tg PS1/APPswe mice compared to wild-type littermates. A significant reduction in Bace1 transcription was observed in Tg PS1/APPswe treated with 20 mg/Kg/Day of anatabine in their drinking water (P<0.001).