Analysis of the binding property of selected recombinant peptides with human sera in an ELISA.

30-mer peptides spanning the WNV-proteins capsid, prM/M and E, fused to GST, were incubated with human sera from outbreaks in Italy (I1-8), Greece (G1–G15) and USA (US1-2). Those peptides displaying signals with an OD >0,5 are shown (the total number of peptides is shown in Figure S1).Values represent the absorption over cut-off (mean of four negative sera plus two standard deviations) and are derived from at least two independent experiments (performed in duplicate). Error bars represent the standard deviation. The background (binding of the serum to GST) was subtracted.