Analysis of serum miRNA expression profiles explored by TLDA.

(A) Correlation and scatter plot analysis of relative miRNAs expression levels in APAP group and vehicle group; (B) Correlation and scatter plot analysis of relative miRNAs expression levels in DB group and vehicle group; (C) Correlation and scatter plot analysis of relative miRNAs expression levels in DB group and APAP group; In Panel A, B and C, the relative expression levels of each miRNA to an internal control was calculated by using the equation 2^−ΔCT×10⁷, in which ΔC_T = C_{T miRNA}−C_{T U6}. The C_T value of “Undetectable” was calculated as 40. (D) Top 10 upregulated and downregulated serum miRNAs detected by TaqMan low-density qPCR array. Fold change of treated/vehicle was calculated by using the equation 2^−ΔΔCT, in which ΔΔC_T = (C_{T miRNA}−C_{T U6}) _{DILI group}−(C_{T miRNA}−C_{T U6}) _{vehicle group}. And serum miRNAs were ranked by the fold change of the group with the largest upregulated fold (n = 10 per group, pooling).