Analysis of serum miRNA expression profiles explored by TLDA.

<p>(A) Correlation and scatter plot analysis of relative miRNAs expression levels in APAP group and vehicle group; (B) Correlation and scatter plot analysis of relative miRNAs expression levels in DB group and vehicle group; (C) Correlation and scatter plot analysis of relative miRNAs expression levels in DB group and APAP group; In Panel A, B and C, the relative expression levels of each miRNA to an internal control was calculated by using the equation 2<sup>−ΔCT</sup>×10<sup>7</sup>, in which ΔC<sub>T</sub> = C<sub>T miRNA</sub>−C<sub>T U6</sub>. The C<sub>T</sub> value of “Undetectable” was calculated as 40. (D) Top 10 upregulated and downregulated serum miRNAs detected by TaqMan low-density qPCR array. Fold change of treated/vehicle was calculated by using the equation 2<sup>−ΔΔCT</sup>, in which ΔΔC<sub>T</sub> = (C<sub>T miRNA</sub>−C<sub>T U6</sub>) <sub>DILI group</sub>−(C<sub>T miRNA</sub>−C<sub>T U6</sub>) <sub>vehicle group</sub>. And serum miRNAs were ranked by the fold change of the group with the largest upregulated fold (n = 10 per group, pooling).</p>