Figure_4.tif (3.27 MB)
Analysis of potential Tre-related genotoxic effects.
figure
posted on 2013-09-26, 02:20 authored by Ilona Hauber, Helga Hofmann-Sieber, Jan Chemnitz, Danilo Dubrau, Janet Chusainow, Rolf Stucka, Philip Hartjen, Axel Schambach, Patrick Ziegler, Karl Hackmann, Evelin Schröck, Udo Schumacher, Christoph Lindner, Adam Grundhoff, Christopher Baum, Markus G. Manz, Frank Buchholz, Joachim Hauber(A) SKY (spectral karyotyping) analysis of metaphase spreads isolated from primary human CD4+ T cells overexpressing Tre for 21 days. An RGB display of the 24-color SKY hybridization of a representative normal metaphase is shown. (B) Array-CGH analysis of DNA isolated from primary human CD4+ T cells overexpressing Tre compared to mock-transfected cells. A representative chromosome (Chr17) is shown. Normal log2 ratios of color intensities (−4 to +4) for each probe populate the chart. A heterozygous deletion would be indicated by a green dot with the value <−1. A heterozygous duplication would be indicated by a red dot with a value >0.66.
