Fig_5.tif (2.19 MB)

Analysis of murine pan-hematopoietic stem cells (CD45⁺-cells) and CD45^- -cells derived from lung (21^st day of the experiment).

figure

posted on 2015-04-30, 03:03 authored by Evgenii Germanovich Skurikhin, Olga Victorovna Pershina, Alena Mikhaylovna Reztsova, Natalia Nikolaevna Ermakova, Ekaterina Sergeevna Khmelevskaya, Vycheslav Andreevich Krupin, Inna Ernestovna Stepanova, Andrew Vladimirovich Artamonov, Andrew Alexandrovich Bekarev, Pavel Gennadjevich Madonov, Alexander Mikhaylovich Dygai

Cells were analyzed by FACS with antibody to identify mouse CD45, CD90.1, CD34, CD73, CD106 (VCAM-1), CD44 and CD31 (PECAM-1) (BD Biosciences, USA). Cells for analysis were derived from lungs of C57BL/6 mice of control and experimental groups. The CD45 negative cells population and CD45 positive cells population were sorted from one sample. The CD45 negative cells population was defined as CD34 and CD31 negative and positive for CD106, CD73, CD44, and CD90.1 at the same time in the two specimens (1 specimen—subpopulation of cells with phenotype CD45^-, CD73⁺, CD90⁺, CD106⁺, CD44⁺ and 2 specimen—subpopulation of cells with phenotype CD45^-, CD73⁺, CD90⁺, CD31^-, CD34^-). Dot plots are representative figures of three independent experiments with the mean from three independent experiments.