Fig_9.tif (4.84 MB)
Analysis of LgAQP1-GFP expression by Western blot.
figure
posted on 2015-02-13, 18:05 authored by Rubens Monte-Neto, Marie-Claude N. Laffitte, Philippe Leprohon, Priscila Reis, Frédéric Frézard, Marc OuelletteAnti-GFP monoclonal antibody was used to confirm the expression of the LgAQP1WT-GFP and LgAQP1G133D-GFP fusion proteins from protein extracts prepared from LgSbIII650.2 parasites transfected with pSP72αZEOαLgAQP1WT (lane 1), pSP72αZEOαLgAQP1G133D (lane 2) or pSP72αZEOα (lane 3). Anti-α-tubulin antiserum was used as a loading control.
