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Allopurinol prevents fructose-induced CHREBP acetylation and nuclear translocation in human hepatocytes.
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posted on 2012-10-24, 01:21 authored by Miguel A. Lanaspa, Laura G. Sanchez-Lozada, Christina Cicerchi, Nanxing Li, Carlos A. Roncal-Jimenez, Takuji Ishimoto, Myphuong Le, Gabriela E. Garcia, Jeffrey B. Thomas, Christopher J. Rivard, Ana Andres-Hernando, Brandi Hunter, George Schreiner, Bernardo Rodriguez-Iturbe, Yuri Y. Sautin, Richard J. JohnsonA) KHK mRNA expression in cells exposed to fructose (5 mmol/L) for different time points. B) Analysis of acetylation state in immunoprecipitated ChREBP in cells exposed to glucose, fructose or mannitiol in the presence or absence of allopurinol. (C–E) ChREBP and KHK expression in nuclear and cytoplasmic extracts of cells control and incubated with glucose (25 mM) and fructose (5 mM) in the presence or absence of allopurinol.
