Agroinfiltration in Z. zerumbet leaves.

Fig 3a. Control leaf of infiltrated Z. zerumbet. Agroinfiltration was carried out by mixing equal amounts of the three Agrobacterium (GV3103) cultures harboring pCaBS-α, pCaBS-β and pCa-γb: 00.The cultures were infiltrated into 2–4 leaves of 3-month Z. zerumbet plants using a 1-ml needleless syringe. The agroinfiltrated plants were maintained in a growth chamber for 30 dpi. Control leaf showed no visual signs of viral infection. Fig 3b. Leaves infiltrated with equal amounts of Agrobacterium cultures harboring pCaBS-α, pCaBS-β and pCaγb:ZzPDS. After 30dpi, yellow stripes were visible along the parallel veins, indicating sites of PDS down-regulation. Fig 3c. A closer view of a PDS-silenced leaf of Z. zerumbet.