Age-related reduction in <i>Apoe</i> expression in the cortex and CA1 is prevented by running.

<p>(A–B) Representative sections and quantitative analysis of <i>Apoe</i>-hybridized cortex on aging <i>Apoe</i><sup><i>-/-</i></sup>, aging, aged sedentary (Sd), and aged runner (Rn) mice showing a significant preservation of <i>Apoe</i> expression on runner mice. No hybridization signal with <i>Apoe</i> riboprobe was observed in the <i>Apoe</i><sup><i>-/-</i></sup> mouse brain. (C–D) Representative sections and quantitative analysis of <i>Apoe</i> expression on aging hippocampal CA1. Down-regulation of <i>Apoe</i> expression in the CA1 region in aging mice is prevented by wheel running. Values in (B and D) are relative mean <u>+</u> SEM to the aging values, <i>n</i> = 6 per group. In (B) *<i>p =</i> 0.0347 and 0.0244, and in (D) **<i>p</i> = 0.0019 and 0.0016 by ANOVA followed by Tukey’s posthoc tests. Scale Bars: 50 μm. The data used to make this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002279#pbio.1002279.s001" target="_blank">S1 Dataset</a>.</p>