Adherence to HeLa cells, and expression of EspA in co-culture relative to pure culture.

<p>(A) Western blot of samples of total cell extracts from pure culture of <i>B</i>. <i>thetaiotaomicron</i> (lane 1), pure culture of EHEC NIPH-11060424 (lane 2), and <i>B</i>. <i>thetaiotaomicron</i> EHEC NIPH-11060424 co-culture (lane 3) using anti-EspA monoclonal antibodies. The arrow represents an unknown protein present in <i>B</i>. <i>thetaiotaomicron</i>. The results shown are representative of three independent biological and technical replicates. (B) The scatter plot shows the adherence of EHEC NIPH-11060424 in co-culture with <i>B</i>. <i>thetaiotaomicron</i> compared to pure culture of EHEC NIPH-11060424 to HeLa cells after 3 and 6 hours of incubation. The data are representative of three independent experiments with 3 technical replicates (n = 9). The vertical line illustrates the median of each group. The Mann-Whitney non-parametric test was used for comparison of groups. P< 0.05 was considered statistically significant.</p>