Figure_5.tif (6.33 MB)

Adenoviral overexpression of AnxA2 in mouse liver significantly increases LDLR levels.

figure

posted on 2012-07-27, 00:33 authored by Nabil G. Seidah, Steve Poirier, Maxime Denis, Rex Parker, Bowman Miao, Claudio Mapelli, Annik Prat, Hanny Wassef, Jean Davignon, Katherine A. Hajjar, Gaétan Mayer

Empty control (Ad-Ctl) and HA-tagged AnxA2 (Ad-A2) adenoviruses were injected intravenously into the tail vein of WT (A) or Pcsk9^−/− (B) mice. After 7 days, livers were analysed for LDLR levels and AnxA2-HA expression by Western blotting. Bars and error bars represent average ± SD. P-values were obtained using a two-tailed Student's t-test. (C) Immunohistochemistry of LDLR and AnxA2-HA in the liver of WT (upper panels) or Pcsk9^−/− (lower panels) mice injected with Ad-Ctl or Ad-A2 adenoviruses. Frozen liver tissue sections were fixed and incubated with anti-LDLR and anti-HA antibodies. Bound primary antibodies were revealed with species-specific Alexa-488 (LDLR, green) and Alexa-555 (HA, red) secondary antibodies. Nuclei were counterstained with DAPI (blue). Arrows show colocalization of LDLR with AnxA2-HA at the cell surface of hepatocytes. Bars = 20 µm.