Action of pretreatments on the changes in efficacy (ΔEmax) of endothelin-1 (ET-1) in human isolated bronchi.

<p>Values were determined in paired human bronchi after incubation with 0.1 µM fenoterol or oxygenated Krebs–Henseleit solution (control) for 15 hours at 37°C with or without pretreatment co-incubated with fenoterol (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111350#pone-0111350-g005" target="_blank">Fig. 5</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111350#pone-0111350-g006" target="_blank">6</a>). Emax (g) represented the maximal contraction induced by 0.1 µM ET-1. ΔEmax represented the difference between Emax obtained with the fenoterol-treated bronchi and Emax obtained with their paired controls. The observed effect of pretreatment co-incubated with fenoterol is small (|<i>d</i>|≥.20), medium (|<i>d</i>|≥.50), or large (|<i>d</i>|≥.80) according to the Cohen's conventions <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0111350#pone.0111350-Cohen1" target="_blank">[36]</a>. The 95% CI for <i>d</i> consists of the uncertainty around the real effect of pretreatment. <sup>†</sup>Except for wortmaninn which was added in organ bath before concentration-response curve for ET-1. *<i>P</i><.05, **<i>P</i><.01, ***<i>P</i><.001 with vs without pretreatment.</p><p>Action of pretreatments on the changes in efficacy (ΔEmax) of endothelin-1 (ET-1) in human isolated bronchi.</p>