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Absence of singlet oxygen in the phototoxic effect of illuminated KR-E. coli.

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posted on 2015-07-27, 02:45 authored by Libo Yan, Masamitsu Kanada, Jinyan Zhang, Shigetoshi Okazaki, Susumu Terakawa

To quantitatively measure the singlet oxygen produced by KillerRed, the intensity of spontaneous luminescence was measured at wavelengths in a near infrared region. (1) KillerRed in PBS. (2) KillerRed in an O2-saturated solution. (3) KillerRed in a deuterium oxide (D2O) solution. (4) Methylene blue solution used as a control. In reference to methylene blue, KillerRed did not produce any singlet oxygen upon irradiation.

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