Aberrant Neural Differentiation of FXS iPSC-derived Neuronal Progenitors.

<p>Immunocytochemical analysis of Tuj1 expression (green) in neuronal progenitor cells differentiated upon mitogen removal of (A) FMRP<sup>+</sup> control line 8330-8 and (B) FMRP<sup>-</sup> FXS line 848-3, overlaid with nuclei DNA staining (blue) (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026203#pone.0026203.s001" target="_blank">Figure S1</a> for additional images). (C) Quantification of neurite process length in indicated iPSC-NP lines. Two wells (6-well plate) were imaged, 9 images per well (n = 18 images per sample). P-values as indicated *1.5e-13, **1.1e-10, ***3.1e-13, ****1.4e-4.</p>