A targeted RNAi screen identifies 17 RNAi candidates that decrease the lifespan of the <i>isp-1;ctb-1</i> mutant.

<p>The mean fold change in lifespan relative to that of wild-type worms treated with empty vector (black bar, value set to 1) was calculated for <i>isp-1;ctb-1</i> mutant treated with empty vector or with each RNAi clone tested. The mean fold changes (± s.d.) presented are averaged from at least three independent experiments. Only the RNAi clones that decrease the lifespan of the <i>isp-1;ctb-1</i> mutant by at least 10% are represented (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001084#pbio-1001084-t001" target="_blank">Table 1</a>). Among them, 13 represent transcription factors not previously known to function in aging and four (indicated with asterisks) are transcription factors already known to affect lifespan in <i>C. elegans</i>. Quantitative data and statistical analyses for the experiments shown here are included in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001084#pbio-1001084-t001" target="_blank">Tables 1</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001084#pbio.1001084.s007" target="_blank">S1</a>.</p>