A. Western blot analysis of adult S. japonicum worm extracts obtained on day 6 following treatment with SjIR dsRNAs.

Results are shown for proteins recognised by anti-Sm-Pmy antibody (top panel), anti-SjLD1 (middle panel) and anti-SjLD2 (bottom panel) antibodies. The intensity of Sm-Pmy expression was evaluated so as to determine equal protein loading. The arrows indicate the diminished level of SjIR proteins relative to the luciferase treatment control in the first lane. The experiment was repeated twice with similar results obtained. B. Western blot analysis showing no immunological cross reactivity between recombinant HIR and the SjLDs. Commercial recombinant human insulin receptor (rHIR) and recombinant SjLD1 (rSjLD1) and SjLD2 (rSjLD2) were electrophoresed on SDS-PAGE gels, blotted to membrane and probed with rabbit anti-HIR polyclonal antibody (left panel), rabbit anti-SjLD1 (middle panel) and anti-SjLD2 (right panel) as primary antibodies with anti-rabbit IgG conjugated to horseradish peroxidise used as secondary antibody.