A–D: Examples of IGF2R allelic expression bias and DMR2 DNA methylation levels for samples from IVF fetuses and controls.

Data points for in-vivo conceived control (AI) fetuses, normal weight in-vitro-fertilization (IVF-n) fetuses and overgrown in-vitro-fertilization (IVF-l) fetuses are shown. (A,C) Allelic expression bias for individual samples from liver and skeletal muscle. The Y axis represents expression from the paternal allele relative to the predominantly expressed maternal allele. (B,D) Corresponding DMR2 methylation levels measured in liver and skeletal muscle of the same individuals. Methylation levels are the mean for 21 CpG sites analyzed by bisulfite pyrosequencing (see Figure 2).