ATP induced cell death through activation of aP2X7R.

<p>(A) Cell death induced by different concentrations of ATP. Macrophages were incubated with PBS (control) or various concentrations of ATP for 30 min and cultured for an additional 6 h without ATP. (B) The effects of aEPAb on ATP-induced cell death. Cells were pre-incubated with different concentrations of aEPAb for 30 min before ATP treatment. (C) The effects of oATP on ATP-induced cell death. The cells were pretreated with various concentrations of oATP for 2 h before ATP treatment. (D) The effects of aP2X7R siRNA on ATP-induced cell death. The cells were transfected with siRNA for 48 h before ATP treatment. Mouse isotype IgG, PBS, and scrambled siRNA were added as controls. Cells were treated with ATP for 30 min, followed by a 6-h incubation in the absence of ATP. Data are representative of three independent experiments. *<i>P</i><0.05; **<i>P</i><0.01.</p>