ATP induced cell death through activation of aP2X7R.

(A) Cell death induced by different concentrations of ATP. Macrophages were incubated with PBS (control) or various concentrations of ATP for 30 min and cultured for an additional 6 h without ATP. (B) The effects of aEPAb on ATP-induced cell death. Cells were pre-incubated with different concentrations of aEPAb for 30 min before ATP treatment. (C) The effects of oATP on ATP-induced cell death. The cells were pretreated with various concentrations of oATP for 2 h before ATP treatment. (D) The effects of aP2X7R siRNA on ATP-induced cell death. The cells were transfected with siRNA for 48 h before ATP treatment. Mouse isotype IgG, PBS, and scrambled siRNA were added as controls. Cells were treated with ATP for 30 min, followed by a 6-h incubation in the absence of ATP. Data are representative of three independent experiments. *P<0.05; **P<0.01.