Fig_5.tif (795.85 kB)
ANGPTL2 induced pro-Inflammatory responses in vitro.
figure
posted on 2015-07-01, 04:45 authored by Yusuke Sasaki, Masayuki Ohta, Dhruv Desai, Jose-Luiz Figueiredo, Mary C. Whelan, Tomohiro Sugano, Masaki Yamabi, Wataru Yano, Tyler Faits, Katsumi Yabusaki, Hengmin Zhang, Andrew K. Mlynarchik, Keisuke Inoue, Ken Mizuno, Masanori AikawaA, Western blotting analysis of FLAG fusion ANGPTL2 protein with anti-FLAG and anti-human Angptl2 antibody (Left: with 2-mercaptoethanol (2-ME), Right: without 2-ME). B, TNF-α concentration in rANGPTL2-treated THP-1 conditioned media (72 hr treatment, n = 3). C, Quantitative RT-PCR of mRNAs encoding Angptl2 and pro-inflammatory related genes in THP-1 cells (24 hr treatment, n = 3). D, Quantitative RT-PCR of mRNAs encoding M2 macrophage markers in THP-1 cells (24 hr treatment, n = 3). Data are mean ± SEM, **: P<0.01, *: P<0.05 compared with BSA group.
