Figure_3.tif (1.67 MB)
2D-immunoblot analysis of CagA and CagA fragments with serum from H. pylori-positive child and CagA-specific antibodies.
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posted on 2014-08-20, 04:06 authored by Junko Akada, Masumi Okuda, Narumi Hiramoto, Takao Kitagawa, Xiulian Zhang, Shuichi Kamei, Akane Ito, Mikiko Nakamura, Tomohisa Uchida, Tomoko Hiwatani, Yoshihiro Fukuda, Teruko Nakazawa, Yasuhiro Kuramitsu, Kazuyuki NakamuraA. See Pico CBB-stained 2-DE gels and immunoblots of CPY2052, HPK5 and NCTC11637 and their cagA deletion strains probed with Hp-positive serum No. 15 serum. B. Immuoblots probed with antibodies for EPIYA motif-containing fragment of western-CagA (anti-western-CagA-C) and East Asian CagA (anti-EAS). Regions of CagA to produce two antibodies are diagrammed schematically. Spot numbers are corresponded to those in Fig. 2. 1(C) or C is CagA, and 2(V) or V is VacA. Spots of a-f or g-k are CagA-fragments in 2-D immunoblot of HPK5 and NCTC11637 lysate, respectively.
