2D-DIGE image of proteins of sarkosyl-insoluble aggregates isolated from the GT81-1C [PSI+][PIN+] strain and its [psi−][pin−] derivative (A).
Spots corresponding to proteins from [PSI+][PIN+] lysate and the [psi−][pin−] control are red and green, respectively, while yellow spots correspond to proteins present in both compared samples. SDS-PAGE image of proteins from the same DRAFs that were insoluble in UTC buffer (B). Proteins identified by mass-spectrometry are indicated. Mr – protein molecular mass markers. Identification data are presented in Table 4; mass spectra are shown in S4 Table.