2D-DIGE image of proteins of SDS- (A) or sarkosyl-insoluble (B) aggregates isolated from the BY4742 [<i>PIN</i><sup>+</sup>] strain expressing Aβ-GFP and from its [<i>pin</i><sup>−</sup>] derivative expressing non-fused GFP.

<p>Spots corresponding to proteins from [<i>PIN</i><sup>+</sup>] cells expressing Aβ-GFP are red, while proteins from GFP-containing cells (control) are green. Yellow spots correspond to proteins present in both compared samples. Proteins identified by mass-spectrometry are indicated. Identification data are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116003#pone-0116003-t001" target="_blank">Table 1</a> (SDS-insoluble aggregates) and 2 and 3(sarkosyl-insoluble aggregates). Mass-spectra of identified proteins are listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116003#pone.0116003.s001" target="_blank">S1</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116003#pone.0116003.s003" target="_blank">S3 Tables</a>, correspondingly.</p>