Y530 is an important phosphorylation site in SGEF.

(A) Cell lysates from HEK293T cells transfected with the indicated plasmids were immunoprecipitated with anti-Flag antibody, and tyrosine phosphorylation (p-Tyr) and total cell lysates were analyzed by immunoblotting with antibodies against phospho-tyrosine, Flag, and HA. (B) Cell lysates from HEK293T cells transfected with the indicated plasmids were incubated with GST-RhoG-G15A, and bound Flag-SGEF and total cell lysates were analyzed by immunoblotting with anti-Flag and anti-HA antibodies. (C) Cell lysates from HEK293T cells transfected with indicated plasmids were incubated with GST-ELMO-NT, and bound Myc-RhoG and total cell lysates were analyzed with antibodies against Myc, Flag, and HA.