Western blot migration analysis of PrP^Sc from 139H, DY and ME7H-infected animals.

Western blot (A) and migration analysis (B, C) of PrP^Sc from 139H, DY or ME7H-infected PK digested brain homogenates at ratios of 1:10, 1:1, or 10:1. In lanes 1, 2, 8, and 9 the unglycosylated PrP^Sc polypeptide migrates at 21 kDa and in lanes 4, 5, and 6 the unglycosylated PrP^Sc polypeptide migrates at 19 kDa. In lane 3 and 7 the migration of the unglycosylated PrP^Sc polypeptide migrates from 19 kDa and 21 kDa. This experiment was repeated a minimum of three times with similar results.