Visualization 4: Robust real-time 3D single-particle tracking using a dynamically moving laser spot

Movie of the 3D-DyPLoT system picking up sequential 100 nm beads, showing both the robustness of the tracking and the ease with which the system can pick up diffusing particles. The inset is the sCMOS readout. The movie is played at 5x real time. Originally published in Optics Letters on 15 June 2017 (ol-42-12-2390)