Visualization 1 – Widefield voltage recordings in neurons

A QuasAr2 voltage recording of cultured rat hippocampal neurons firing action potentials in response to CheRiff stimulus. The average grayscale image is displayed throughout the movie, and the activity is overlaid using a red (small depolarizations) to yellow (large depolarizations) color map. The blue square in the lower left indicates when stimulation with blue light occurs. The 0.5 x 4 mm field of view was recorded at 1000 frames per second (fps) and is played back at 30 fps.