Visualization 1 - mouse brain imaging results with ICG injection.mp4

Visualization 1 shows the non-invasive time lapse imaging results of the mouse brain in vivo. Fluorescence and optoacoustic data were acquired for a total duration of 300 s, starting ~10 s prior to the ICG injection. Data from the first 30 s is shown here. During the experiment, the laser was set to a wavelength of 700 nm and a pulse repetition rate of 25 Hz. The camera integration time was set to 0.1 s. A long pass filter (Edmund Optics, Stock # 66-227) with 800 nm cut-on wavelength was placed in front of the camera to acquire the fluorescence images.