ab6b00532_si_001.pdf (578.97 kB)
Viruslike Particles Encapsidating Respiratory Syncytial Virus M and M2 Proteins Induce Robust T Cell Responses
journal contribution
posted on 2016-11-03, 00:00 authored by Benjamin Schwarz, Kaitlyn M. Morabito, Tracy J. Ruckwardt, Dustin P. Patterson, John Avera, Heini M. Miettinen, Barney S. Graham, Trevor DouglasSubunit
vaccines provide a safe, focused alternative to conventional
vaccines. However, these vaccines often require significant adjuvants
and
are particularly hard to target toward cytotoxic T lymphocyte (CTL)
immunity. Viruslike particles (VLPs) provide biomaterial scaffolds
with pathogen-like polyvalent structures making them useful platforms
for biomimetic antigen delivery to the immune system. Encapsidation
of antigens within VLPs has been shown to enhance antigen availability
for CD8 T cell responses. Here, we examine the potential to generate
complex responses to multiple subunit antigens localized within the
same VLP particle. Two proteins of respiratory syncytial virus (RSV)
with well-characterized CD8 T cell responses, the matrix (M) and matrix
2 (M2) proteins, were successfully coencapsidated within the P22 VLP.
Upon intranasal administration in mice, the particles stimulated CD8
T cell memory responses against both antigens. In addition, vaccination
elicited tissue-resident T cell populations. Upon subsequent RSV challenge,
P22-M/M2-treated mice displayed significantly reduced lung viral titers.
This demonstrates the utility of the P22 VLP in directing immune responses
to multiple encapsidated viral antigens, demonstrating the potential
of this technology to facilitate immunity to multiple targets simultaneously.
History
Usage metrics
Categories
Keywords
biomimetic antigen deliverywell-characterized CD 8 T cell responsesM 2 Proteins Induce Robust T Cell Responses Subunit vaccinespathogen-like polyvalent structuresVLP particleantigen availabilityCD 8 T cell responsesbiomaterial scaffoldsCTLP 22 VLPcytotoxic T lymphocytesyncytial virusP 22-M miceViruslike Particles Encapsidating Respiratory Syncytial Virus MRSV challengesubunit antigenstissue-resident T cell populationsCD 8 T cell memory responsesmatrix 2Viruslike particles
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC