Viability of Met-5A and primary mesothelial cells exposed to <i>S</i>. <i>epidermidis</i> and lipoteichoic acid.

<p>Confluent Met-5A mesothelial cells were exposed to 10<sup>7</sup> cfu/mL of two <i>S</i>. <i>epidermidis</i> reference isolates (ATCC<sup>®</sup> 14990 and ATCC<sup>®</sup> 12228), five <i>S</i>. <i>epidermidis</i> clinical isolates from PD peritonitis patients (C015, C016, C017, C018, C019) and 10 μg/mL lipoteichoic acid (LTA) for 1 hour at 37°C. Confluent primary mesothelial cells were exposed to 10<sup>7</sup> cfu/mL of the clinical <i>S</i>. <i>epidermidis</i> isolate C016. Viability was determined using flow cytometry and a LIVE/DEAD<sup>®</sup> Fixable Near-IR Dead Cell Stain, and data reported as the mean percentage of cell death across a minimum of biological triplicates (error bars are standard deviation). There was no statistically significant (<i>p</i> < 0.05) difference in the percent of cell death between any of the samples and the media control.</p>