Validation of Venlafaxine in Pharmaceutical Dosage by Reverse Phase HPLC

A rapid, specific and accurate isocratic HPLC method was developed and validated for the assay of venlafaxine in pharmaceutical dosage forms. The assay
involved an isocratic – elution of venlafaxine in ODS- C18 column using mobile phase composition consists of (65:35 v/v) of acetonitrile and sodium acetate
with 0.1% tri-ethyl amine. The wavelength of detection is 225nm.The method showed good linearity in the range of 2.0-50.0 mg/mL. The runtime of the
method is 5 mins. The proposed method can be used for routine quality control samples in industry in bulk and in finished dosage forms. In present study,
a rapid specific precise and validated HPLC method for the quantitative estimation of venlafaxine in pharmaceutical dosage forms has been reported. The
developed method can be applied to directly and easily to the analysis of the pharmaceutical tablet p reparations. The percentage recoveries were near
100% for givenmethods. The method was completely validated and proven to be rugged. The excipients did not interfere in the analysis. The results showed
that this method can be used for rapid determination of venlafaxine in pharmaceutical tablet with precision, accuracy and specificity.