Fig 6.tif (512.56 kB)
V1aR inhibitor reduces astrocyte damage and cellular AQP4 expression.
figure
posted on 2017-12-07, 18:58 authored by Jonghyuk Park, Takahiro Masaki, Yoshihiro Mezaki, Hiroshi Yokoyama, Mariko Nakamura, Haruka Maehashi, Takahiko J. Fujimi, Sabine S. Gouraud, Keisuke Nagatsuma, Madoka Nakagomi, Naofumi Kimura, Tomokazu Matsuura(A) Hoechst 33342 and PI double-staining in astrocytes. Cells were treated with ACT, combination of ACT and AVP, and combination of ACT and AVP with OPC-21268 for 48 h. (B) Percentages of cell death were calculated by determining the ratio of PI-stained cells to Hoechst stained cells. (C) The expression of AQP4 mRNA at 48 h after ACT, combination of ACT and AVP, and combination of ACT and AVP with OPC-21268 were analyzed by quantitative real-time RT-PCR. Bars indicate the mean ± standard error of the mean (n = 3). *p < 0.05, in comparison to control, # p < 0.05.
