V1aR inhibitor reduces astrocyte damage and cellular AQP4 expression.

<p>(A) Hoechst 33342 and PI double-staining in astrocytes. Cells were treated with ACT, combination of ACT and AVP, and combination of ACT and AVP with OPC-21268 for 48 h. (B) Percentages of cell death were calculated by determining the ratio of PI-stained cells to Hoechst stained cells. (C) The expression of AQP4 mRNA at 48 h after ACT, combination of ACT and AVP, and combination of ACT and AVP with OPC-21268 were analyzed by quantitative real-time RT-PCR. Bars indicate the mean ± standard error of the mean (<i>n</i> = 3). *<i>p</i> < 0.05, in comparison to control, # <i>p</i> < 0.05.</p>