Urinary beta-trace protein gene expression analysis in type 2 diabetes mellitus patients

dataset

posted on 2018-01-24, 02:41 authored by Marcelo Rodrigues Bacci, Beatriz da Costa Aguiar Alves, Marina Romera Cavallari, Ligia Ajaime Azzalis, Ross Martin de Rozier-Alves, Matheus Moreira Perez, Ethel Zimberg Chehter, Edimar Cristiano Pereira, Fernando Luiz Affonso Fonseca

ABSTRACT Objective: To evaluate the gene expression of beta-trace protein in urine of diabetic patients, with no reduction in glomerular filtration rate, which was defined as below 60mL/min/1.73m2. Methods: Type 2 diabetes mellitus patients were recruited, and a group of non-diabetic individuals served as control. Beta-trace protein gene expression was analyzed by quantitative PCR. Blood samples were collected to establish glucose levels and baseline kidney function. Accuracy was analyzed using ROC curves. Results: Ninety type 2 diabetes mellitus patients and 20 non-diabetic individuals were recruited. The area under the curve was 0.601, sensitivity of 20% and specificity of 89.47%. Among diabetic participants, 18% showed an expression above the cutoff point. Conclusion: These results of accuracy of beta-trace protein gene expression in urine of diabetic patients are promising, although they did not achieve a higher area under the curve level.