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Tumor growth in nude mice after Dox-induced expression of shRNA/Plk1MM or shRNA/Plk1
figure
posted on 2011-12-31, 02:53 authored by Sven Kappel, Yves Matthess, Brigitte Zimmer, Manfred Kaufmann, Klaus StrebhardtCopyright information:
Taken from "Tumor inhibition by genomically integrated inducible RNAi-cassettes"
Nucleic Acids Research 2006;34(16):4527-4536.
Published online 31 Aug 2006
PMCID:PMC1636372.
© 2006 The Author(s)
Mice were inoculated with 1 × 10 HeLa cell clones in each flank HeLa clones for inducible expression of shRNA/Plk1MM were inoculated on the left flank whereas clones for inducible expression of shRNA/Plk1 were inoculated on the right flank. () Relative tumor volumes of xenografted US-type HeLa tumors for inducible expression of shRNA/Plk1MM or shRNA/Plk1 either after induction with Dox or in the absence of Dox starting 23 days after inoculation. Significant inhibition of tumor growth ( < 0.05) is indicated with an asterisk. () Northern blot analysis of Plk1 mRNA under the influence of shRNA/Plk1MM or shRNA/Plk1 expression. Plk1 mRNA expression in US-type promoter-derived HeLa tumors of mice without Dox-treatment (lanes 1 and 3) in comparison to tumors of mice treated with Dox (lanes 2 and 4). For standardization ethidiumbromide staining of 28S rRNA was chosen. Significant reduction ( < 0.05) is indicated with an asterisk.