Trichostatin A attenuated lung stretch-induced fibrogenic biomarkers.
Five days after bleomycin treatment, representative photomicrographs (x400) with E-cadherin (E-cad, red or bright green), α-smooth muscle actin (α-SMA, red), and Hoechst (blue) immunofluorescent staining of paraffin lung sections (A) (n = 5 per group), and micro-computed tomography imaging (B) (n = 3 per group) were from nonventilated control mice and mice ventilated at a tidal volume of 6 mL/kg or 30 mL/kg for 5 h with room air. TSA 2 mg/kg was given intraperitoneally 1 h before mechanical ventilation. Positive red and bright green staining in the lung epithelium and interstitium is identified by arrows (n = 5 per group). The fibrotic scoring (C) was quantified as the average number of 10 nonoverlapping fields in Masson’s trichrome staining of paraffin lung sections (n = 5 per group). Scale bars represent 20 μm. ANOVA was used to assess the statistical significance of the differences, followed by multiple comparisons with a Scheffeˊs test, and a P value < 0.05 was considered statistically significant. * P< 0.05 versus the nonventilated control mice with bleomycin pretreatment; † P< 0.05 versus all other groups.