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Transplanted hMNPs promote histological recovery and alter intracellular signaling pathways.

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posted on 29.07.2010 by Sharyn L. Rossi, Gabriel Nistor, Tanya Wyatt, Hong Zhen Yin, Aleksandra J. Poole, John H. Weiss, Matthew J. Gardener, Sipke Dijkstra, David F. Fischer, Hans S. Keirstead

(a) hMNP transplantation enhanced sprouting of endogenous serotonergic (5-HT) projections. hMNP-transplanted animals consistently contained aberrant projections throughout the dorsal gray matter (top panels, arrows) and dense innervation of the ventral horns (bottom panels) at 2 mm cranial to the injury site (b) At 2 mm and 3 mm cranial to the injury epicenter, and 1 mm caudal to the injury epicenter, 5-HT immunoreactivity was significantly greater than that observed in control animals. At 1 mm cranial to the injury epicenter, and 2 mm and 3 mm caudal to the injury epicenter, 5-HT immunoreactivity was not significantly different than in control animals. (c) NeuN immunostaining demonstrated that hMNP transplantation enhanced survival of endogenous (human nuclear antigen-negative) neurons 2 mm cranial to the injury site. (d) Quantification of enhanced neuronal survival in hMNP-transplanted animals cranial and caudal to the injury site. (e) hMNP transplantation attenuated phosphorylation of stress-associated protein kinase (SAPK). (f) Densitometric quantification of SAPK normalized to actin controls showed that 1 and 4 days following transplantation, phosphorylation of SAPK decreased in hMNP-transplanted animals relative to controls; no significant differences were observed between groups at 7 and 10 days. Bar = 200 µm for (a), 100 µm for (c).

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