Transgenic expression of 3xHA-Digitor-mCitrine in salivary gland cells.

(A) Diagram of 3xHA-Digitor-mCitrine. (B) Polytene squash preparation labeled with anti-HA antibody to detect the 3xHA-Digitor-mCitrine construct (in green) and with Hoechst to label DNA (in blue/grey). In the composite image (comp) examples of developmental puff regions with higher expression of 3xHA-Digitor-mCitrine are indicated by arrowheads. (C) Polytene squash preparation labeled with anti-HA antibody to detect the 3xHA-Digitor-mCitrine construct (in green) and with anti-Skeletor antibody (in red). In the composite image (comp) co-localization between 3xHA-Digitor-mCitrine and Skeletor is indicated by yellow/orange colors. Examples of developmental puff regions positive for 3xHA-Digitor-mCitrine but not for Skeletor are indicated by arrowheads. The relatively low exposure of anti-HA antibody-labeling of 3xHA-Digitor-mCitrine in (B) shows the prominent labeling of the developmental puffs whereas the longer exposure in (C) highlights the labeling of the weaker labeled interband regions.