nl9b02483_si_001.pdf (1.34 MB)
Transformation of Viral Light Particles into Near-Infrared Fluorescence Quantum Dot-Labeled Active Tumor-Targeting Nanovectors for Drug Delivery
journal contribution
posted on 2019-09-11, 18:38 authored by Cheng Lv, Tian-Yu Zhang, Yi Lin, Man Tang, Cai-Hua Zhai, Hou-Fu Xia, Ji Wang, Zhi-Ling Zhang, Zhi-Xiong Xie, Gang Chen, Dai-Wen PangNanosized oncolytic
viral light particles (L-particles), separated
from progeny virions, are composed of envelopes and several tegument
proteins of viruses, free of nucleocapsids. The noninfectious L-particles
experience the same internalization process as mature oncolytic virions,
which exhibits great potential to act as targeted therapeutic platforms.
However, the clinical applications of L-particle-based theranostic
platforms are rare due to the lack of effective methods to transform
L-particles into nanovectors. Herein, a convenient and mild strategy
has been developed to transform L-particles into near-infrared (NIR)
fluorescence Ag2Se quantum dot (QD)-labeled active tumor-targeting
nanovectors for real-time in situ imaging and drug
delivery. Utilizing the electroporation technique, L-particles can
be labeled with ultrasmall water-dispersible NIR fluorescence Ag2Se QDs with a labeling efficiency of ca.
85% and loaded with antitumor drug with a loading efficiency of ca. 87%. Meanwhile, by harnessing the infection mechanism
of viruses, viral L-particles are able to recognize and enter tumor
cells without further modification. In sum, a trackable and actively
tumor-targeted theranostics nanovector can be obtained efficiently
and simultaneously. Such multifunctional nanovectors transformed from
viral L-particles have exhibited excellent properties of active tumor-targeting, in vivo tumor imaging, and antitumor efficacy, which opens
a new window for the development of natural therapeutic nanoplatforms.
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noninfectious L-particles experienceoncolytic virionstegument proteinstumor-targeted theranostics nanovectorNear-Infrared Fluorescence Quantum Dot-Labeledfluorescence Ag 2 Se quantum dotvivo tumor imagingprogeny virionstumor-targeting nanovectorsinternalization processultrasmall water-dispersible NIR fluorescence Ag 2 Se QDsinfection mechanismelectroporation techniqueantitumor drugL-particle-based theranostic platformslight particlestumor cellsSuch multifunctional nanovectorsDrug Delivery Nanosized oncolyticViral Light Particlesantitumor efficacyloading efficiencydrug deliveryTumor-Targeting Nanovectors
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