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Transcriptome Landscape of Porcine Intramuscular Adipocytes during Differentiation
journal contribution
posted on 2017-07-04, 00:00 authored by Delin Mo, Kaifan Yu, Hu Chen, Luxi Chen, Xiaohong Liu, Zuyong He, Peiqing Cong, Yaosheng ChenThe adipocyte differentiation process,
controlled by a tightly
regulated transcriptional cascade, contributes partly to determine
intramuscular adipose tissue (IMAT) mass, which is associated with
meat quality in food animals, as well as obesity and related metabolic
complications in human. Thus, this study aimed to characterize genes
critical for intramuscular preadipocyte differentiation. Primary intramuscular
preadipocytes were isolated from pigs, and mRNA profiles were performed
at several key points (0 h, 4 h, 8 h, 1 day, 2 days, and 6 days) during
adipogenesis using microarrays. By gene functional analysis, we identified
numerous differentially expressed genes among distinct stages of intramuscular
preadipocyte differentiation, which included numbers of transcription factors
in the early stages. We obtained 4 clusters of differential
gene expression pattern, including crucial candidate genes associated
with adipogenesis of intramuscular adipocytes. Further, we demonstrated that POSTN and FGFR4 suppressed, whereas AKR1CL1 promoted,
the expression of adipogenic marker PPARγ and C/EBPα. Taken together, our data delineated the transcriptome
landscape during porcine intramuscular preadipocyte differentiation,
which provided a valuable resource for finding the genes responsible
for IMAT formation.
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mRNA profilesintramuscular adipocytesadipogenic marker PPAR γPOSTN4 hcandidate genesPrimary intramuscular preadipocytesintramuscular preadipocyte differentiationAKR 1CLintramuscular adipose tissuetranscription factorsIMAT formation2 daystranscriptional cascade4 clustersporcine intramuscular preadipocyte differentiationmeat qualityTranscriptome Landscapegene expression patternadipocyte differentiation processtranscriptome landscapeFGFR 41 day6 daysfood animalsPorcine Intramuscular Adipocytes8 h
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