pr500890n_si_001.pdf (2.01 MB)
Total Body Exposure to Low-Dose Ionizing Radiation Induces Long-Term Alterations to the Liver Proteome of Neonatally Exposed Mice
journal contribution
posted on 2015-01-02, 00:00 authored by Mayur
V. Bakshi, Omid Azimzadeh, Zarko Barjaktarovic, Stefan J. Kempf, Juliane Merl-Pham, Stefanie
M. Hauck, Sonja Buratovic, Per Eriksson, Michael J. Atkinson, Soile TapioTens of thousands of people are being
exposed daily to environmental
low-dose gamma radiation. Epidemiological data indicate that such
low radiation doses may negatively affect liver function and result
in the development of liver disease. However, the biological mechanisms
behind these adverse effects are unknown. The aim of this study was
to investigate radiation-induced damage in the liver after low radiation
doses. Neonatal male NMRI mice were exposed to total body irradiation
on postnatal day 10 using acute single doses ranging from 0.02 to
1.0 Gy. Early (1 day) and late (7 months) changes in the liver proteome
were tracked using isotope-coded protein label technology and quantitative
mass spectrometry. Our data indicate that low and moderate radiation
doses induce an immediate inhibition of the glycolysis pathway and
pyruvate dehydrogenase availability in the liver. Furthermore, they
lead to significant long-term alterations in lipid metabolism and
increased liver inflammation accompanying inactivation of the transcription
factor peroxisome proliferator-activated receptor alpha. This study
contributes to the understanding of the potential risk of liver damage
in populations environmentally exposed to ionizing radiation.