Tissue samples used for ribosome profiling.

2016-07-14T15:35:03Z (GMT) by Prakitchai Chotewutmontri Alice Barkan
<p><b>(A)</b> Leaf segments analyzed in this study. The indicated segments were excised from leaf 3 of nine-day-old seedlings. The segments are numbered according to the nomenclature of [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006106#pgen.1006106.ref013" target="_blank">13</a>]. <b>(B)</b> Immunoblots showing abundance of marker proteins in the leaf gradient tissue used for ribosome profiling. Replicate immunoblots were probed with antibodies to subunits of the chloroplast ATP synthase (AtpB), cytochrome <i>b</i><sub><i>6</i></sub><i>f</i> complex (PetD), Photosystem I (PsaD), Photosystem II (D1), the NDH-like complex NdhH), chloroplast ribosomes (Rpl2), and mitochondrial ATP synthase (Atp6). Samples were loaded on the basis of equal protein. An image of one blot stained with Ponceau S (below) serves as a loading control and illustrates the abundance of the large subunit of Rubisco (RbcL). PT, plastid proteins; MT, mitochondrial protein. <b>(C</b>) Replicate immunoblots showing abundance of marker proteins in mesophyll (M) and bundle sheath (BS) fractions used for ribosome profiling. Samples were loaded on the basis of equal protein. An image of one blot stained with Ponceau S (below) illustrates the abundance of several marker proteins. PEPC, phosphoenolpyruvate carboxylase; PPDK, pyruvate orthophosphate dikinase; ME, NADP-dependent malic enzyme; other abbreviations as in panel (B).</p>