Timeline of events.

Three weeks after plating, when neural networks were considered mature, cortical cell cultures were treated with 100 μM αS. Within the first day after αS addition spontaneous synaptic activity rose while electrically induced network response started to decrease. Within two days after addition, spontaneous activity and the number of electrodes on which activity was recorded dropped below baseline. At day five, on average, about one third of the cells had an inclusion body. By day six hardly any spontaneous activity could be recorded and electrical stimulation was no longer able to evoke a network response.