Time course of PNGase F treatment of IgA1.

Narimatsu, Yoshiki; Atsushi, Kuno; Ito, Hiromi; Kaji, Hiryoyuki; Kaneko, Shyuzo; Yamagata, Kunihiro; Narimatsu, Hisashi

doi:10.6084/m9.figshare.894412.v1

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Time course of PNGase F treatment of IgA1.

journal contribution

posted on 2014-02-14, 06:11 authored by Yoshiki NarimatsuYoshiki Narimatsu, Kuno Atsushi, Hiromi Ito, Hiryoyuki Kaji, Shyuzo Kaneko, Kunihiro Yamagata, Hisashi Narimatsu

IgA1 purified from the sera of the mIgA-MIDD patient (A) and an MPCD patient (B) sera was digested partially with PNGase F for the indicated times (lanes 1–5, 7–10). Complete digestion of N-glycans was performed by incubation with PNGase F for 120 min (lanes 6, 11). Arrowheads indicate the position of PNGase F.